Cell surface characterization of T lymphocytes and allergen-specific T cell clones: Correlation of CD26 expression with T "H"1 subsets

Autor: Willheim, M., Ebner, C., Baier ^a, K., Kern, W., Schrattbauer, K., Thein^a, R., Kraft, D., Breiteneder, H., Reinisch, W., Scheiner, O.
Zdroj: The Journal of Allergy and Clinical Immunology; September 1997, Vol. 100 Issue: 3 p348-355, 8p
Abstrakt: To evaluate the possibility of using surface molecules as markers for human T helper cell subsets, we studied the expression of surface molecules on T cell clones (TCCs) specific for the major birch pollen allergen, Bet v 1. No difference in the expression of the respective receptors for interferon-@c (IFN-@c), IL-4, IL-6, or IL-10 could be detected on T "H subsets, nor did CD25 expression (IL-2R @a-chain) differ significantly. However, high expression of CD26 antigen (dipeptidyl peptidase IV) correlated with a T "H"1/T "H"0-like phenotype, whereas T "H"2-like clones displayed a lower expression of CD26 antigen. Comparing cytokine production and CD26 expression simultaneously, we found a correlation between the IL-4/IFN-@c ratio and the density of CD26 per cell. We could show that the amount of IL-4 secretion, and not of IFN-@c secretion, was responsible for this correlation. To evaluate whether CD26 antigen expression is regulated by stimuli inducing a T "H"1- or T "H"2-like phenotype, peripheral blood mononuclear cells (PBMC) were cultured in the presence of IL-4, IFN-@c, and IL-12, respectively. Incubation with IL-4 led to T cells with a T "H"2-like cytokine pattern and a significantly lower expression of CD26; IFN-@c and IL-12 led to a T "H"1 shift associated with an increased expression of CD26 on CD4^+ T cells. By means of intracellular cytokine detection we analyzed expression of CD26 on CD4^+ PBMC stimulated to produce IFN-@c or IL-4 on a single cell level. All activated, cytokine-producing CD4^+ T cells expressed CD26, but the increase in CD26 expression was higher in cells producing IFN-@c. These data suggest that regulation of CD26 cell surface expression correlates with the production of T "H"1-like cytokines. (J Allergy Clin Immunol 1997;100:348-55.)
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