| Autor: |
Post, JC, White, GJ, Aul, JJ, Zavoral, T, Wadowsky, RM, Zhang, Y, Preston, RA, Ehrlich, GD |
| Zdroj: |
Molecular Diagnosis; January 1996, Vol. 1 Issue: 1 p29-39, 11p |
| Abstrakt: |
Background:Conventional simplex polymerase chain reaction (PCR)-based assays are limited in that they only provide for the detection of a single infectious agent. Many clinical diseases, however, present in a nonspecific, or syndromic, fashion, thereby necessitating the simultaneous assessment of multiple pathogens. Panel-based molecular diagnostic testing can be accomplished by the development of multiplex PCR-based assays, which can detect, individually or severally, different pathogens that are associated with syndromic illness. As part of a larger program of panel development, an assay that can simultaneously detect Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis was developed. These organisms were chosen as they are the most common bacterial pathogens associated with both the acute and chronic forms of otitis media; they are also responsible for a high percentage of sinus infections in both children and adults. In addition, H. influenzae and S. pneumoniae are commonly associated with septic meningitits. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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