| Autor: |
M. Rose, Tyler, D. Prestwich, Glenn |
| Zdroj: |
Organic Letters; June 2006, Vol. 8 Issue: 12 p2575-2578, 4p |
| Abstrakt: |
Fluorogenic analogues of phosphatidylcholine and lysophosphatidylcholine, DDPBand lysoDDPB, were synthesized by an enzyme-assisted strategy. The analogues were evaluated as substrates for phospholipases C and D and lysophospholipase D. DDPBwas cleaved by bacterial and plant phospholipase D (PLD) enzymes and represents the first direct fluorogenic substrate for real-time measurement of PLD activity. Both fluorogenic substrates have potential in screening for PLD and PC−PLC inhibitors and for monitoring spatiotemporal changes in PLD activity in cells. |
| Databáze: |
Supplemental Index |
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