| Autor: |
Ehrhardt, Christina, Schmolke, Mirco, Matzke, Andreas, Knoblauch, Alexander, Will, Carola, Wixler, Viktor, Ludwig, Stephan |
| Zdroj: |
Signal Transduction : Receptors, Mediators and Genes; June 2006, Vol. 6 Issue: 3 p179-184, 6p |
| Abstrakt: |
In search for a cheap and effective transfection reagent we used the positively charged polyplex-forming compound polyethylenimine (PEI). This compound is commercially available from different companies either as a non-modified chemical reagent or with additives as a more cost intensive transfection reagent. Here we used the non-modified PEI reagent to optimize transfection protocols for different cell-lines. With these optimized conditions we were able to transiently transfect a number of cell-lines up to 40–90%. |
| Databáze: |
Supplemental Index |
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