| Autor: |
Richards, Gillian R., Smith, Alison J., Parry, Frances, Platts, Amy, Chan, Grace K.Y., Leveridge, Mathew, Kerby, Julie E., Simpson, Peter B. |
| Zdroj: |
Assay and Drug Development Technologies; April 2006, Vol. 4 Issue: 2 p143-152, 10p |
| Abstrakt: |
The prospect of manipulating endogenous neural stem cells to replace damaged tissue and correct functional deficits represents a novel mechanism for treating a variety of central nervous system disorders. Using human neural precursor cultures and a variety of assays for studying stem cell behavior we have screened two libraries of commercially available compounds using an endpoint high content screening assay. We then performed detailed follow-up mechanistic studies on confirmed hits using endpoint and kinetics assays to characterize and differentiate the mechanisms of action of these compounds. The screening cascade employed successfully identified a number of active compounds with differing mechanisms of action. This approach shows how hits from a phenotypic screen can be prioritized and characterized by high content screening to identify potentially novel mechanisms and druggable targets to take forward into more conventional highthroughput screeening approaches. |
| Databáze: |
Supplemental Index |
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