| Autor: |
AGHAJANIAN, Suren, WORRALL, D.Margaret |
| Zdroj: |
Biochemical Journal; July 2002, Vol. 365 Issue: 1 p13-18, 6p |
| Abstrakt: |
The final two enzymes in the CoA biosynthetic pathway, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), are separate proteins in prokaryotes, but exist as a bifunctional enzyme in pig liver. In the present study we have obtained sequence information from purified pig-liver enzyme, and identified the corresponding cDNA in a number of species. The human gene localizes to chromosome 17q12-21 and contains regions with sequence similarity to the monofunctional Escherichia coli DPCK and PPAT. The recombinant 564-amino-acid human protein confirmed the associated transferase and kinase activities, and gave similar kinetic properties to the wild-type pig enzyme. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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