| Autor: |
Akimoto, S., Ono, T., Tsutsui, H., Kinouchi, T., Kataoka, K., Ohnishi, Y. |
| Zdroj: |
Biochemical and Biophysical Research Communications; September 1994, Vol. 203 Issue: 2 p914-921, 8p |
| Abstrakt: |
The nucleotide sequence of the Bacteroides fragilisneuraminidase-encoding gene (nanH) and its flanking regions was determined. The B. fragilis nanHopen reading frame (ORF) of 1632 nucleotides encoded a neuraminidase of 544 amino acid residues with a calculated molecular mass of 59.51 kDa. The amino acid sequence at the N-terminus of the deduced protein exhibited the common features of procaryotic signal sequences. The Asp box sequence, Ser-X-Asp-X-Gly-X-Thr-Trp, which is conserved among bacterial neuraminidases, was found at five positions in the deduced amino acid sequence. A single transcription start point was identified 88 nucleotides upstream of the nanHORF. Northern blot analysis using an internal nanHprobe revealed a single mRNA species with a length of 1.8 kb that could encode just the neuraminidase. |
| Databáze: |
Supplemental Index |
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