| Autor: |
Luss, Hartmut, Nussler, Natascha C., Beger, Hans-G., Nussler, Andreas K. |
| Zdroj: |
Methods: A Companion to Methods in Enzymology; August 1996, Vol. 10 Issue: 1 p51-60, 10p |
| Abstrakt: |
Three different isoforms of the enzyme nitric oxide synthase (NOS) (EC 1.14.13.39) catalyze the formation of nitric oxide (NO) froml-arginine, which is then converted tol-citrulline. NO released by the constitutive isoforms is involved in a variety of physiologic functions, whereas larger amounts of NO released from the inducible isoform (iNOS) are mostly associated with inflammatory processes. Overproduction of NO in these processes including sepsis and autoimmune diseases can have deleterious consequences and pathophysiologic relevance. In this regard investigations of the regulation and function of iNOS to find specific iNOS inhibitors to block unwanted high levels of NO seem of great interest. The present article gives an overview of several methods and techniques employed to study the expression and regulation of the inducible nitric oxide synthase inin vivoandin vitromodels of inflammation. The induction of iNOS was detected at different levels of expression and was compared to functional activity of NOS measured as enzyme activity and nitrite/nitrate production, two stable end products of the NO pathway. Differencesin vivoandin vitroare compared and discussed. |
| Databáze: |
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