Crystallization and Preliminary X-ray Diffraction Studies on ADP-ribosylation Factor 1

Autor: Greasley, Samantha, Jhoti, Harren, Fensome, Amanda C., Cockcroft, Shamshad, Thomas, Geraint M.H., Bax, Ben
Zdroj: JMB Online (Journal of Molecular Biology); December 15, 1994, Vol. 244 Issue: 5 p651-653, 3p
Abstrakt: ADP-ribosylation factor 1 (ARF-1) is a member of a family of small G-proteins that regulate both intracellular vesicle transport and phospholipase D activity. Crystals of ARF-1 suitable for X-ray diffraction analysis have been grown in the presence of GDP by the hanging drop vapour diffusion method. Crystal grows in space C2 with cell dimensions a = 122.36 Å, b = 45.01 Å, c = 91.96 Å and β = 133.62° and diffract to at least 2.3 Å resolution. A second crystal form has been characterized (space group C2, a = 69.70 Å, b = 45.25 Å, c = 60.45 Å, β = 109.6°) but does not grow reproducibly. Copyright 1994, 1999 Academic Press
Databáze: Supplemental Index