Crystallization and Preliminary X-ray Analysis of (1,3)- and (1,3;1,4)-β-dcl025;-Glucanases from Germinating Barley

Autor: Chen, Lin, Garrett, Thomas J.P., Varghese, Jose N., Fincher, Geoffrey B., Høj, Peter B.
Zdroj: JMB Online (Journal of Molecular Biology); December 1993, Vol. 234 Issue: 3 p888-889, 2p
Abstrakt: (1,3)-β-d-Glucanase isoenzyme GII and (1,3;1,4)-β-d-glucanase isoenzyme EII from barley have been crystallized by the hanging drop method in the presence of ammonium sulphate. The crystals of the (1,3)-β-d-glucanase, which diffract to about 1·8 Å resolution, belong to the trigonal space group P31212 (or P32212) with cell constants a= b= 86·9 Å c= 156·0 Å and contain two molecules in the asymmetric unit. The crystals of the (1,3;1,4)-β-dglucanase which diffract to better than 1·8 Å resolution, belong to the tetragonal space group P43212 (or P41212) with cell constants a= b= 87·4 Å, c= 109·5 Å and contain one molecule in the asymmetric unit.
Databáze: Supplemental Index