| Autor: |
Merritt, William D., Taylor, Barbara J., Der-Minassian, Violette, Reaman, Gregory H. |
| Zdroj: |
Cellular Immunology; October 1996, Vol. 173 Issue: 1 p131-148, 18p |
| Abstrakt: |
The ganglioside GD3is preferentially expressed on the surface of malignant T cell lymphoblasts and on resting T cells which express the memory cell phenotype, CD45RA−CD29+. However, GD3expression in activated T cells and its potential function in proliferating normal and malignant T cells are unclear. Utilizing three-color immunostaining and flow cytometry, we examined changes in the expression of GD3in conjunction with the RA and RO isoforms of CD45 duringin vitroT cell activation. GD3was equally expressed in resting CD4 and CD8 cells and was specifically found in the CD45RO+RA−population. Activation of T cells with PHA resulted in an increased percentage of GD+3cells. This increase was evident by the first day and was observed in the CD45RO−(naive cell) population; by 2 days, GD3was expressed heterogeneously in a large population of CD45RO+RA+cells. Further activation of T cells with PHA or anti-CD3 monoclonal antibody (OKT3) resulted in a further increase in GD3-expressing cells, and the increase in GD3density correlated with increased CD45RO and loss of CD45RA. In contrast, increases in GD3and interleukin-2 receptor (CD25) expression in response to PHA or OKT3 occurred independently, indicating that the GD3/CD45RO coexpression observed was not a general consequence of cell activation. The results provide evidence for specific comodulation of GD3and CD45RO during T cell mitogenesis, and thus suggest that these molecules may colocalize on the T cell surface. |
| Databáze: |
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