| Abstrakt: |
The ability of two strains ofLactobacillus acidophilus, CRL 640 and CRL 800, to survive and retain their biological activities under frozen storage was determined. Freezing and thawing, as well as frozen storage, damaged the cell membrane, rendering the microorganisms sensitive to sodium chloride and bile salts. Both lactic acid production and proteolytic activity were depressed after 21 days at −20°C, whereas β-galactosidase activity per cell unit was increased. Cell injury was partially overcome after repair in a salt-rich medium. |
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