Differential Scanning Calorimetry and Hydrodynamic Study of Bacterial Viruses. Effect of solution conditions

Autor: Mrevlishvili, G. M., Sottomayor, M. J., Ribeiro da Silva, M. A. V., Mdzinarashvili, T. D., Al-Zaza, M., Tediashvili, M., Tushishvili, D., Chanishvili, N.
Zdroj: Journal of Thermal Analysis and Calorimetry; October 2001, Vol. 66 Issue: 1 p103-113, 11p
Abstrakt: Bacterial viruses genome- ds-DNA -expulsion from the phage capsid is induced by temperature and is not accompanied by heat effects (temperature interval 45–75°C). Thus the temperature induced ejectionof genetic material from phages is predominantly entropic. ds-DNA output from the capsid increases the viscosity of the phage suspension at least 100 times. ds-DNA output from the capsid is accompanied by a significant change of partial volume. The disruption of 1mg of phage produces ΔV=1.3×10–9m3which corresponds to a volume increase of 200%. This produces exothermic heat effects in closed calorimetric cells, with free volume above the measured liquid. This paper deals with the study of the V-group, family T-even, E. Coli bacteria phage(named unphage). According to electron microscopic pictures, the geometrical parameters of this phage are 750×560 Å (from head) and 900 Å (from tail).
Databáze: Supplemental Index
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