Induction of Pathogenic Anti-dsDNA Antibodies Is Controlled on the Level of B Cells in a Non-Lupus Prone Mouse Strain

Autor: Langnickel, Dirk, Enghard, Philipp, Klein, Claudia, Undeutsch, Reinmar, Hocher, Berthold, Manz, R., Burmester, G., Riemekasten, Gabriela
Zdroj: Journal of Clinical Immunology; January 2006, Vol. 26 Issue: 1 p86-95, 10p
Abstrakt: The SmD183–119 peptide is a main target of autoantibodies and T cells in human and murine lupus, but its role in autoimmunity induction remains elusive. Therefore, female Balb/c mice and (NZW × Balb/c)F1 [CWF1] mice with identical MHC haplotype as lupus prone NZB/W mice were immunized with SmD183−119. Immunizations of CWF1 mice with SmD183–119, but not with the controls (irrelevant peptide, HEL peptide, or saline), induced anti-SmD183–119 and anti-dsDNA antibodies and proteinuria not present in Balb/c mice. DsDNA-specific plasma cell induction after SmD183–119 immunizations was confirmed by ELISPOT assays showing that the generation of dsDNA-specific antibody forming cells (AFC) was mainly driven by increased T-cell help. T-cell help for the generation of dsDNA-specific AFC was also present in saline-treated CWF1 mice but was controlled on the levels of B cells preventing autoimmunity.
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