| Autor: |
Clausse, Bernard, Fizazi, Karim, Walczak, Véronique, Tetaud, Cécile, Wiels, Joëlle, Tursz, Thomas, Busson, Pierre |
| Zdroj: |
Virology; February 1997, Vol. 228 Issue: 2 p285-293, 9p |
| Abstrakt: |
Latent Membrane Protein 1 (LMP1) is an EBV-transforming protein which is detected both in lymphoblastoid cell lines—resulting from EBV-immortalizationin vitro—and in undifferentiated nasopharyngeal carcinoma (NPC), an EBV-associated malignancy of epithelial origin. To better define LMP1 subcellular targets, LMP1 distribution was analyzed in cellular glycosphingolipid-rich complexes (GSL-complexes) derived from epithelial and lymphoid cells. These complexes are obtained by extraction of glycosphingolipid-rich membrane domains (GSL-domains), which are clustering sites for heterotrimeric G-proteins and G-protein-associated receptors. LMP1 concentration was enriched 50-fold in GSL-complexes extracted from a NPC tumor line, C15. High concentrations of LMP1 were also observed in GSL-complexes derived from cultured lymphoid and epithelial cells. These data suggest that association with GSL-domains is an important step in LMP1 trafficking and is probably required for some aspects of its biological activity. |
| Databáze: |
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