Determination of DNA in Densovirus-Infected Invertebrate Cell Line by Flow Cytometry

Autor: Odier, F., Vago, P., Quiot, J. M., Devauchelle, G., Bureau, J. P.
Zdroj: Journal of Invertebrate Pathology; November 1993, Vol. 62 Issue: 3 p252-256, 5p
Abstrakt: The DNA content of lepidopteran cell lines infected with densovirus was determined by flow cytometry. Vital DNA production was monitored and in vitro viral cytopathogenic effects were analyzed. The increased fluorochrome binding was linked to an increase in viral DNA content and to alteration in the nucleus structure observed under electron microscopy. The information provided by flow cytometry was complemented by ultrastructural studies. Copyright 1993, 1999 Academic Press
Databáze: Supplemental Index