| Autor: |
Hogg, Dayle S., Albarwani, Sulayma, Davies, Andrew R.L., Kozlowski, Roland Z. |
| Zdroj: |
Biochemical and Biophysical Research Communications; September 1999, Vol. 263 Issue: 2 p405-409, 5p |
| Abstrakt: |
We have, for the first time, developed a reliable method for freshly isolating viable endothelial cells from resistance-sized rat pulmonary arteries. The endothelial origin of these cells was confirmed using indirect immunofluorescence, utilizing fluorescently labeled low-density lipoprotein. Biophysical and pharmacological patch-clamp experiments conducted under quasiphysiological cationic gradients revealed that these cells had a mean resting membrane potential of ∼−38 mV and displayed a delayed-rectifying K+current. Immunohistochemical staining of rat lung cross-sections revealed an abundance of KV1.5 channel protein in pulmonary endothelium. This is the first report of a delayed-rectifying K+current in endothelial cells of resistance-sized pulmonary arteries. Since changes in membrane potential associated with K+channel activity affect release of vasoactive substances from endothelial cells, this finding has important implications for understanding the mechanisms underlying control of pulmonary vascular tone. |
| Databáze: |
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