| Autor: |
Logan, Karen M, Skiba, Mark C, Eldin, Sherif, Knight, Kendall L |
| Zdroj: |
JMB Online (Journal of Molecular Biology); February 1997, Vol. 266 Issue: 2 p306-316, 11p |
| Abstrakt: |
We have analyzed the oligomeric properties of a number of mutant RecA proteins containing single amino acid substitutions within one region of the subunit interface. In contrast to wild-type RecA, which forms a heterogeneous population of different-sized oligomers, we find that many of these mutant proteins exist in a more homogeneous oligomeric form, which approximates to the size of a RecA hexamer. Some of these mutants have a significant level of activity in vivofor recombinational DNA repair and thus represent the first mutant RecA proteins identified which retain activity yet can exist in a discrete oligomeric state as free protein. |
| Databáze: |
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| Externí odkaz: |
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