| Autor: |
Abe, Koichiro, Hashiyama, Motohiro, Macgregor, Grant, Yamamura, Ken-ichi, Abe, Kuniya |
| Zdroj: |
Developmental Biology; December 1996, Vol. 180 Issue: 2 p468-472, 5p |
| Abstrakt: |
Tissue nonspecific alkaline phosphatase (TNAP), the product of theAkp2locus, is expressed in mouse primordial germ cells (PGC) for an extensive period during embryogenesis. Mice with theAkp2tm1Sormutant allele of TNAP expresslacZ(β-galactosidase; β-gal) under control of theAkp2locus. PGCs were purified fromAkp2tm1Sorembryos using fluorescence activated cell sorting of β-gal expressing cells (FACS-gal). Analysis of the purified cells by alkaline phosphatase staining and immunocytochemistry with anti-c-kitantibody demonstrated that highly (98%) purified PGCs can be isolated using this method. This technique will facilitate experiments that require highly purified preparations of PGCs including cell culture and gene expression analyses. |
| Databáze: |
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