| Autor: |
Khmel, I. A., Ovadis, M. I., Mayatskaya, A. V., Veselovskii, A. M., Bass, I. A., Lipasova, V. A., Bolshoy, A., Chet, I., Chernin, L. S. |
| Zdroj: |
Journal of Basic Microbiology; December 2005, Vol. 45 Issue: 6 p426-437, 12p |
| Abstrakt: |
To study the regulation of expression of the Serratia plymuthica gene chiA encoding a 58-kDa endochitinase, its 586-bp-long upstream regulatory region was cloned, sequenced and fused to a promoterless lac operon in phage λRS45 to obtain a single-copy transcriptional fusion (PF1chiA-lac ) in lysogens of Escherichia coli wild-type strains or their mutants deficient in various global regulators of transcription. The level of PF1chiA-lac expression increased about 20- and 90-fold, respectively, in E. coli K12 Δhns and double Δhns stpA mutants deficient in H-NS, and in both H-NS and StpA DNA-binding histone-like proteins, as compared to levels in the wild-type strain. In a Δlrp mutant deficient in the leucine-responsive transcriptional regulator Lrp, the level of PF1chiA-lac expression increased only up to threefold, whereas even smaller differences relative to the wild-type strain were observed in rpoS and Δcrp mutants deficient in the ωS subunit of RNA polymerase and catabolite-repression protein (CRP), respectively. Deletion of the inverted-repeat sequences and curved DNA regions located in the upstream region of chiA essentially did not influence strain IC1270's chiA promoter activity in E. coli . (© 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) |
| Databáze: |
Supplemental Index |
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