| Autor: |
Leaver, H.A., Craig, S.R., Yap, P.L., Williams, J.R., Walker, W.S. |
| Zdroj: |
Biologicals; December 1996, Vol. 24 Issue: 4 p319-324, 6p |
| Abstrakt: |
Reactive oxygen (roi) generation was investigated in phagocyted of 39 patients undergoing pulmonary resection for lung cancer and 39 paired healthy controls. Generation of roi in monocytes and neutrophils was monitored using 2′, 7′-dichlorofluorescein diacetate. Activation associated with hydrophobic interactions was probed by analysis of phagocyte roi activation by arachidonic acid and γ-linolenic acid. Patient roi was measured pre-operatively and 2 and 7 days post-operatively. Elevated (P<0.01) roi productin was detected in neutrophils of lung cancer patients. surgery was associated with an increase (P<0.05–P<0.01) in phagocyte roi at 2 and 7 days post-op. Phagocyte roi was stimulated by arachidonic acid and γ-linolenic acid (1–40 μM) both pre- and post-operatively. Differences in arachidonic acid and γ-linolenic acid stimulation between patient and control and pre- and post-op patient phagocyted suggest arachidonic acid involvement in phagocyte activation during reactive responses to lung carcinoma and surgery. |
| Databáze: |
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| Externí odkaz: |
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