| Autor: |
Hoizey, Guillaume, Dukic, Sylvain, Vistelle, Richard, Lamiable, Denis, Millart, Hervé, d’Arbigny, Pierre |
| Zdroj: |
Analyst; December 1, 1999, Vol. 124 Issue: 12 p1761-1764, 4p |
| Abstrakt: |
A sensitive gas chromatographic–mass spectrometric (GC-MS) procedure is described for the selective determination of gacyclidine (a non-competitive N-methyl-D-aspartate antagonist) in rat plasma and spinal cord dialyzates. It involves a single-step liquid–liquid extraction of plasma samples and dialyzates with hexane (pH 8.0) and the use of phencyclidine as an internal standard. The compounds were separated on a GC capillary column and specifically detected by MS in the selected-ion monitoring mode. Gacyclidine and its internal standard were monitored by using the fragment ions at m/z 206 and 200, respectively. The method was accurate and reproducible (intra- and inter-day reproducibility <12%) with a limit of quantification of 1.6 ng ml–1 using 100 µl plasma of dialyzate samples. The calibration curves for rat plasma and Ringer’s solution were linear (r2 > 0.996) over a range from 1.6 to 200 ng ml–1. The extraction efficiency was close to 100%. This simple and rapid assay (total run time <10 min) was validated for a pilot pharmacokinetic study in healthy rats after intravenous injection of a bolus dose of gacyclidine (2.5 mg kg–1). |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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