| Autor: |
Lewis, A., Hartness, M. E., Chapman, C. G., Fearon, I. M., Meadows, H. J., Peers, C., Kemp, P. J. |
| Zdroj: |
Biochemical and Biophysical Research Communications; August 3, 2001, Vol. 285 Issue: 5 p1290-1294, 5p |
| Abstrakt: |
Hypoxic inhibition of background K+ channels is crucial to O2 sensing by chemoreceptor tissues, but direct demonstration of O2 sensitivity by any member of this K+ channel family is lacking. HEK293 cells were transfected with a pcDNA3.1-hTASK1 construct; expression of hTASK1 was verified using RT-PCR and immunocytochemistry. Whole-cell K+ currents of cells stably expressing hTASK-1 were, as anticipated, extremely sensitive to extracellular pH, within the physiological range (IC50 ≈ 7.0). All cells expressing this signature pH sensitivity were acutely modulated by pO2; reduction of pO2 from 150 to <40 mmHg (at pH 7.4) caused rapid and reversible suppression of pH-sensitive K+ currents. Furthermore, these two regulatory signals clearly acted at the same channel, since the magnitude of the O2-sensitive current was dependent on the extracellular pH. These data represent the first direct verification that hTASK1 is O2-sensitive and reinforce the idea that this K+ channel is key to O2 sensing in chemoreceptors. |
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