| Autor: |
Peters, Maire, Jõgi, Eerik, Suitso, Indrek, Punnisk, Tiina, Nurk, Allan |
| Zdroj: |
Plasmid; July 2001, Vol. 46 Issue: 1 p25-36, 12p |
| Abstrakt: |
We describe features of the basic replicon of the 10.6-kb medium-copy-number plasmid pAM10.6. pAM10.6 was able to replicate in various Pseudomonasstrains but was maintained in Escherichia colionly after the p15A origin of replication was inserted. Deletion analysis suggests that the pAM10.6 origin of replication is located in a 0.5-kb region that includes inverted and direct repeats upstream of the repAgene. RepA (204 aa) has a clear homology to plasmid replication proteins of some other gram-negative bacteria. The pas(plasmid addiction system) (genes encoded in the region of 480-bp) stabilizes plasmid maintenance in P. putidacells under nonselective conditions for at least 200 generations. A 3.75-kb PstI fragment of pAM10.6 joined to a Kmrgene was shown to be a minimal plasmid unit maintained in P. putidaas a monomer. Further deletions of this 3.75-kb fragment caused a drive to form stable head-to-tail dimeric plasmids in P. putida. |
| Databáze: |
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