| Autor: |
Ying, Z., Agarwal, K.C., Beylot, M., Soloviev, M.V., David, F., Reider, M., Tserng, K.Y., Brunengraber, H. |
| Zdroj: |
Analytical Biochemistry; August 1, 1993, Vol. 212 Issue: 2 p481-486, 6p |
| Abstrakt: |
We present gas chromatographic-mass spectrometric assays for (i) the concentration of sulfamethoxazole and (ii) the concentration and molar percentage enrichment of acetyl-sulfamethoxazole in biological fluids. The compounds are extracted with ethyl acetate, derivatized with either diazomethane or pentafluorobenzyl bromide, and analyzed by gas chromatography-mass spectrometry. Quantitation is achieved using internal standards, [2H4]sulfamethoxazole and acetyl-[2H4]sulfamethoxazole. Limits of detection are 200 nmol for the methyl derivatives and 2 nmol for the pentafluorobenzyl derivatives. The high sensitivity of the assay with the pentafluorobenzyl derivatives allows measuring in plasma and urine (i) the pharmacokinetics of sulfamethoxazole and acetyl-sulfamethoxazole and (ii) the stable isotope enrichment of the acetyl moiety of acetyl-sulfamethoxazole. The latter is used as a probe for the noninvasive chemical biopsy of liver extramitochondrial acetyl-CoA.Copyright 1993, 1999 Academic Press |
| Databáze: |
Supplemental Index |
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