| Autor: |
Martinson, T.A., Plumley, F.G. |
| Zdroj: |
Analytical Biochemistry; June 1995, Vol. 228 Issue: 1 p123-130, 8p |
| Abstrakt: |
Photosynthetic pigments and acyl lipids were simultaneously extracted and concentrated by sec-butanol. Pigments extracted with sec-butanol were indistinguishable from those extracted using acetone as determined by quantitative and qualitative HPLC. Use of sec-butanol has several advantages over conventional extraction solvents: (1) pigments are extracted directly from polyacrylamide gel slices without an elution step; (2) pigments in dilute, isolated pigment-protein complexes are extracted and concentrated without first concentrating the sample; (3) when necessary, the concentration factor is readily increased by addition of water; (4) sec-butanol extracts acyl lipids and vitamin K1as effectively, but much quicker, than chloroform:methanol; (5) sec-butanol rapidly extracts and concentrates pigments from thylakoids of all plant species tested and even directly from many algal/higher plant cells, facilitating analysis of pigment biosynthetic pathways using radioactive substrates; and (6) pigments are stable in sec-butanol for several days at room temperature in the dark or for many weeks if stored at −20°C in darkness. Finally, sec-butanol is preferable to ether for concentrating pigments extracted with acetone. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full Text from ScienceDirect