| Autor: |
Ashour, Abdussalam Ali Ramadan, Birchal Collares, Guilherme, Nogueira Mendes, Edilberto, o, Queiroz, es, es, Teles de Carvalho, Anfrisina Sales, de Oliveira, Celso Affonso, Ferreira Nogueira, Ana Margarida Miguel, Aguiar Rocha, Gifone, Rocha, Andreia Maria Camargos |
| Zdroj: |
Journal of Clinical Microbiology; May 2001, Vol. 39 Issue: 5 p1746-1750, 5p |
| Abstrakt: |
ABSTRACTData concerning the geographic distribution of iceAalleles are scarce, and information on the association of the gene with the disease is rare and still controversial. Furthermore, no such study has been developed in Brazil, where duodenal ulcer and gastric adenocarcinoma are very common. We investigated, by PCR, the frequency of iceAalleles and cagAstatus inHelicobacter pyloristrains isolated from 142 patients (62 children and 80 adults; 66 female; mean age, 30.0 years; age range, 3 to 78 years) with gastritis, duodenal ulcer, or gastric adenocarcinoma.iceAwas identified in bacterium samples obtained from all patients. Eleven (7.7%) of them were infected with multiple strains. Among the patients with nonmixed infection, iceA2allele was detected in 118 (90.1%). iceA2allele was associated with ulcer (P= 0.02) and with carcinoma (P= 0.001). iceA2amplicons of 229, 334, or 549 bp were detected, but none of them was associated with the patient's disorder. iceA2strains were more frequent in patients older than 7 years (P= 0.001). The gene was also more frequent in strains obtained from males (P= 0.02). cagAwas more common in strains obtained from carcinoma (P= 0.0008) and ulcer patients (P< 0.006). cagA-positive strains were more frequent in children older than 7 years (P< 0.003). No association between cagAstatus and sex was found (P= 0.28). In conclusion, we thinkiceAshould not be used as a reliable marker for predicting the clinical outcome of H. pyloriinfection. |
| Databáze: |
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