| Autor: |
Gomez, Arley, Mve-Obiang, Armand, Vray, Bernard, Rudnicka, Wieslawa, Shamputa, Isdore C., Portaels, Franc¸oise, Meyers, Wayne M., Fonteyne, Pierre-Alain, Realini, Laurence |
| Zdroj: |
Journal of Clinical Microbiology; April 2001, Vol. 39 Issue: 4 p1396-1401, 6p |
| Abstrakt: |
ABSTRACTPhospholipase C plays a key role in the pathogenesis of several bacterial infections, for example, those caused by Clostridium perfringensand Listeria monocytogenes. Previous studies have reported multiple copies of plcgenes homologous to Pseudomonas aeruginosa plcHandplcNgenes encoding the hemolytic and nonhemolytic phospholipase C enzymes in the genomes of Mycobacterium tuberculosis, M. marinum, M. bovis, and M. ulcerans. In this study we analyzed the possible relationship between phospholipase C and hemolytic activity in 21 strains of nontuberculous mycobacteria representing nine different species. Detection of phospholipase C enzymatic activity was carried out using thin-layer chromatography to detect diglycerides in the hydrolysates of radiolabeled phosphatidylcholine. DNA sequences of M. kansasiiand M. marinumhomologous to the genes encoding phospholipase C from M. tuberculosisand M. ulceranswere identified by DNA-DNA hybridization and sequencing. Finally, we developed a direct and simple assay to detect mycobacterial hemolytic activity. This assay is based on a modified blood agar medium that allows the growth and expression of hemolysis of slow-growing mycobacteria. Hemolytic activity was detected in M. avium, M. intracellulare, M. ulcerans, M. marinum, M. tuberculosis,andM. kansasiimycobacteria with phospholipase C activity, but not in M. fortuitum. No hemolytic activity was detected inM. smegmatis, M. gordonae, and M. vaccae. Whether or not phospholipase C enzyme plays a role in the pathogenesis of nontuberculous mycobacterial diseases needs further investigation. |
| Databáze: |
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