| Autor: |
Figueiredo, Ce´u, Quint, Wim, Nouhan, Nathalie, van den Munckhof, Henk, Herbrink, Paul, Scherpenisse, Joost, de Boer, Wink, Schneeberger, Peter, Perez-Perez, Guillermo, Blaser, Martin J., van Doorn, Leen-Jan |
| Zdroj: |
Journal of Clinical Microbiology; April 2001, Vol. 39 Issue: 4 p1339-1344, 6p |
| Abstrakt: |
ABSTRACTHelicobacter pyloristrains can be distinguished by genotyping of virulence-associated genes, such as vacAandcagA. Because serological discrimination between strain types would reduce the need for endoscopy, 61 patients carryingH. pyloriwere studied by vacAandcagAgenotyping of H. pyloriin gastric biopsy specimens and by detection of specific serum antibodies. Serological responses to H. pyloriwere determined by Helicoblot (versions 2.0 and 2.1). Antibodies to CagA also were determined by a rapid anti-CagA assay (Pyloriset screen CagA) as well as by two noncommercially developed enzyme immunoassays, each using a recombinant CagA protein. Assessment of performance of the Helicoblot assays indicated substantial interobserver variation, with kappa values between 0.20 and 0.93. There was no relationship between the serological profiles on the Helicoblot and the genotypes from the same patients, except for strong associations between the presence of anti-CagA and the cagA-positive andvacAs1 H. pylorigenotypes. Detection of anti-CagA by the five different assays varied considerably, with kappa values ranging from 0.21 to 0.78. Using the cagAgenotype as the “gold standard,” the sensitivity and specificity of the anti-CagA assays varied from 71.4 to 85.7% and from 54.2 to 100%, respectively. Thus, serological profiles of antibodies to H. pyloriare heterogeneous and, with the exception of anti-CagA antibodies, show no relation to the H. pylori vacAandcagAgenotypes. Detection of anti-CagA antibodies is strongly dependent on the test used. |
| Databáze: |
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