| Autor: |
Suffys, P. N., da Silva Rocha, A., de Oliveira, M., Dias Campos, C. E., Werneck Barreto, A. M., Portaels, F., Rigouts, L., Wouters, G., Jannes, G., van Reybroeck, G., Mijs, W., Vanderborght, B. |
| Zdroj: |
Journal of Clinical Microbiology; December 2001, Vol. 39 Issue: 12 p4477-4482, 6p |
| Abstrakt: |
ABSTRACTINNO-LiPA Mycobacteria (LiPA; Innogenetics, Zwijnaarde, Belgium) is a kit for the simultaneous detection and identification ofMycobacteriumspecies in culture and identifies theMycobacterium tuberculosiscomplex, the M. aviumcomplex (MAC), and the following Mycobacteriumspecies: M. kansasii,M. avium,M. intracellulare,M. scrofulaceum,M. gordonae,M. xenopi, and the M. chelonae-M. abscessuscomplex. The assay, which targets the 16S-23S rRNA spacer region, was evaluated on 157 mycobacterial strains that had been identified by conventional techniques and PCR-restriction enzyme analysis of the hsp65gene (PRA). Forty-seven reference strains consisting of 37 different species and 110 human clinical isolates were submitted to the test, and all were hybridized with theMycobacteriumgenus probe (MYC) on the LiPA strip (100% sensitivity). Ninety-four isolates hybridized to their corresponding species- or complex-specific probes; only one isolate phenotypically identified as M. gordonaedid not react with its specific probe (99.4% accuracy). Thirty-seven MAC strains were phenotypically identified to the complex level and to the species level by LiPA asM. avium(n= 18) or M. intracellulare(n= 7) or as belonging to theM. avium-M. intracellulare-M. scrofulaceumcomplex (n= 12). Of the last 12 strains, 10 had M. aviumPRA patterns and 2 had M. intracellularePRA patterns. Three isolates that had been identified as a single species by conventional identification were proven to be mixed cultures by the LiPA assay. The whole procedure can be performed in 1 working day, starting with the supernatant of a small amount of bacterial mass that had been treated by freezing and then boiling. |
| Databáze: |
Supplemental Index |
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