| Autor: |
Gafan, Gavin P., Lucas, Victoria S., Roberts, Graham J., Petrie, Aviva, Wilson, Michael, Spratt, David A. |
| Zdroj: |
Journal of Clinical Microbiology; September 2004, Vol. 42 Issue: 9 p4141-4146, 6p |
| Abstrakt: |
ABSTRACTPorphyromonas gingivalis, Actinobacillus actinomycetemcomitans, and Tannerella forsythensishave been implicated as the main etiological agents of periodontal disease. The purpose of this work was to estimate the prevalence of these organisms in plaque from children without gingivitis (group 1; n= 65) and from those with gingivitis (group 2; n= 53). Extracted DNA from plaque was subjected to two rounds of PCR targeting the 16S rRNA gene using both universal primers and species-specific primers. The results were as follows: group 1, P. gingivalis, 49%; A. actinomycetemcomitans, 55%; and T. forsythensis, 65%; group 2, P. gingivalis, 47%; A. actinomycetemcomitans, 59%; and T. forsythensis, 45%. T. forsythensiswas detected more frequently in children with no gingivitis than in those with gingivitis (P= 0.03). There was no significant difference between the two groups with respect to the presence of P. gingivalisor A. actinomycetemcomitansin either group (P> 0.05). Logistic regression analysis revealed that the odds of a patient having gingivitis were 2.3 times greater in the absence of T. forsythensis. In conclusion, the results of this study have shown that the three pathogens can be detected in the dental plaque of healthy children and of those with gingivitis and that T. forsythensisis associated with dental plaque at sites with no gingivitis. |
| Databáze: |
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