| Autor: |
Kremer, Kristin, Glynn, Judith R., Lillebaek, Troels, Niemann, Stefan, Kurepina, Natalia E., Kreiswirth, Barry N., Bifani, Pablo J., van Soolingen, Dick |
| Zdroj: |
Journal of Clinical Microbiology; September 2004, Vol. 42 Issue: 9 p4040-4049, 10p |
| Abstrakt: |
ABSTRACTMycobacterium tuberculosisBeijing genotype strains are highly prevalent in Asian countries and in the territory of the former Soviet Union. They are increasingly reported in other areas of the world and are frequently associated with tuberculosis outbreaks and drug resistance. Beijing genotype strains, including W strains, have been characterized by their highly similar multicopy IS6110restriction fragment length polymorphism (RFLP) patterns, deletion of spacers 1 to 34 in the direct repeat region (Beijing spoligotype), and insertion of IS6110in the genomic dnaA-dnaNlocus. In this study the suitability and comparability of these three genetic markers to identify members of the Beijing lineage were evaluated. In a well-characterized collection of 1,020 M. tuberculosisisolates representative of the IS6110RFLP genotypes found in The Netherlands, strains of two clades had spoligotypes characteristic of the Beijing lineage. A set of 19 Beijing reference RFLP patterns was selected to retrieve all Beijing strains from the Dutch database. These reference patterns gave a sensitivity of 98.1% and a specificity of 99.7% for identifying Beijing strains (defined by spoligotyping) in an international database of 1,084 strains. The usefulness of the reference patterns was also assessed with large DNA fingerprint databases in two other European countries and for identification strains from the W lineage found in the United States. A standardized definition for the identification of M. tuberculosisstrains belonging to the Beijing/W lineage, as described in this work, will facilitate further studies on the spread and characterization of this widespread genotype family of M. tuberculosisstrains. |
| Databáze: |
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