| Autor: |
Bavykin, Sergei G., Lysov, Yuri P., Zakhariev, Vladimir, Kelly, John J., Jackman, Joany, Stahl, David A., Cherni, Alexey |
| Zdroj: |
Journal of Clinical Microbiology; August 2004, Vol. 42 Issue: 8 p3711-3730, 20p |
| Abstrakt: |
ABSTRACTIn order to determine if variations in rRNA sequence could be used for discrimination of the members of the Bacillus cereusgroup, we analyzed 183 16S rRNA and 74 23S rRNA sequences for all species in the B. cereusgroup. We also analyzed 30 gyrBsequences for B. cereusgroup strains with published 16S rRNA sequences. Our findings indicated that the three most common species of the B. cereusgroup, B. cereus, Bacillus thuringiensis, and Bacillus mycoides, were each heterogeneous in all three gene sequences, while all analyzed strains of Bacillus anthraciswere found to be homogeneous. Based on analysis of 16S and 23S rRNA sequence variations, the microorganisms within the B. cereusgroup were divided into seven subgroups, Anthracis, Cereus A and B, Thuringiensis A and B, and Mycoides A and B, and these seven subgroups were further organized into two distinct clusters. This classification of the B. cereusgroup conflicts with current taxonomic groupings, which are based on phenotypic traits. The presence of B. cereusstrains in six of the seven subgroups and the presence of B. thuringiensisstrains in three of the subgroups do not support the proposed unification of B. cereusand B. thuringiensisinto one species. Analysis of the available phenotypic data for the strains included in this study revealed phenotypic traits that may be characteristic of several of the subgroups. Finally, our results demonstrated that rRNA and gyrBsequences may be used for discriminating B. anthracisfrom other microorganisms in the B. cereusgroup. |
| Databáze: |
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