| Autor: |
Brooks, Brian W., Robertson, Ruth H., Lutze-Wallace, Cheryl L., Pfahler, Wolfram |
| Zdroj: |
Infection and Immunity; December 2001, Vol. 69 Issue: 12 p7596-7602, 7p |
| Abstrakt: |
ABSTRACTMonoclonal antibodies (MAbs) to the lipopolysaccharide (LPS) O-antigens of Campylobacter fetusserotype A and B strains were produced. Eight MAbs specific for serotype A LPS were characterized on immunoblots of C. fetusserotype A LPS. Two immunoblot patterns were observed and were used to divide the eight MAbs into two groups. MAbs M1177 and M1194 were selected as representative of the two groups and were used in an enzyme-linked immunosorbent assay (ELISA) to examine the LPS O-antigen epitopes of 37 serotype A C. fetussubsp. fetusand C.fetussubsp. venerealisstrains. Thirty-three strains (89%) reacted with both M1177 and M1194, 2 strains reacted only with M1177, and 2 strains reacted only with M1194. To further characterize the O-antigen epitopes, purified serotype A LPS was treated using various temperature and pH conditions and the effect of the treatments on the reactivity of the LPS with MAbs M1177 and M1194 was evaluated by ELISA. While no difference among several treatments was observed, heating serotype A LPS under alkaline conditions decreased the reaction with M1177 to background levels and increased the reaction with M1194. MAbs M1177 and M1194 were also used with ELISA to investigate in vivo and in vitro expression of the two O-antigen epitopes. There was substantial variation in expression of the two epitopes among weekly isolates of two C. fetusserotype A strains recovered from experimentally infected heifers. There was minimal variation in expression of the two epitopes in successive subcultures of three C. fetusserotype A strains. |
| Databáze: |
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