| Autor: |
Allioli, Nathalie, Thomas, Jean-Luc, Chebloune, Yahia, Nigon, Victor-Marc, Verdier, Gérard, Legras, Catherine |
| Zdroj: |
Developmental Biology; September 1994, Vol. 165 Issue: 1 p30-37, 8p |
| Abstrakt: |
Three methods of isolating primordial germ cells (PGCs) from gonads of 5-day-old chick embryos were compared. PGCs were then cultured in vitroin DMEM/F12 medium containing 10% fetal calf serum. BrdU incorporation showed that at least 10% of the PGC population were dividing, under our culture conditions, during the 2nd day of in vitroculture. During this culture period, PGCs were exposed to avian leukosis sarcoma virus-based retrovital vector pseudotyped with subgroup A envelope, carrying the LacZreporter gene. X-Gal staining showed that PGCs were permissive to infection, with more than 50% of PGCs expressing the β-Gal protein. These data represent the first demonstration that PGCs, isolated from gonads of 5-day-old chick embryos, are able to divide in vitroand that it is possible to introduce and express exogenous DNA in chick PGCs maintained in vitro. |
| Databáze: |
Supplemental Index |
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