| Autor: |
Tonui, Willy K., Mejia, J. Santiago, Hochberg, Lisa, Mbow, M. Lamine, Ryan, Jeffrey R., Chan, Adeline S. T., Martin, Samuel K., Titus, Richard G. |
| Zdroj: |
Infection and Immunity; October 2004, Vol. 72 Issue: 10 p5654-5661, 8p |
| Abstrakt: |
ABSTRACTThe potential of Leishmania majorculture-derived soluble exogenous antigens (SEAgs) to induce a protective response in susceptible BALB/c mice challenged with L. majorpromastigotes was investigated. Groups of BALB/c mice were immunized with L. majorSEAgs alone, L. majorSEAgs coadministered with either alum (aluminum hydroxide gel) or recombinant murine interleukin-12 (rmIL-12), L. majorSEAgs coadministered with both alum and rmIL-12, and L. majorSEAgs coadministered with Montanide ISA 720. Importantly and surprisingly, the greatest and most consistent protection against challenge with L. majorwas seen in mice immunized with L. majorSEAgs alone, in the absence of any adjuvant. Mice immunized with L. majorSEAgs had significantly smaller lesions that at times contained more than 100-fold fewer parasites. When lymphoid cells from L. majorSEAg-immunized mice were stimulated with leishmanial antigen in vitro, they proliferated and secreted a mixed profile of type 1 and type 2 cytokines. Finally, analyses with Western blot analyses and antibodies against three surface-expressed and secreted molecules of L. major(lipophosphoglycan, gp46/M2/PSA-2, and gp63) revealed that two of these molecules are present in L. majorSEAgs, lipophosphoglycan and the molecules that associate with it and gp46/M2/PSA-2. |
| Databáze: |
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