| Autor: |
Brahimi, Karima, Badell, Edgar, Sauzet, Jean-Pierre, BenMohamed, Lbachir, Daubersies, Pierre, Guérin-Marchand, Claudine, Snounou, George, Druilhe, Pierre |
| Zdroj: |
Infection and Immunity; June 2001, Vol. 69 Issue: 6 p3845-3852, 8p |
| Abstrakt: |
ABSTRACTThe Plasmodium falciparumliver-stage antigen 3 (LSA3), a recently identified preerythrocytic antigen, induces protection against malaria in chimpanzees. Using antibodies from individuals with hyperimmunity to malaria affinity purified on recombinant or synthetic polypeptides of LSA3, we identified four non-cross-reactive B-cell epitopes in Plasmodium yoeliipreerythrocytic stages. On sporozoites the P. yoeliiprotein detected has a molecular mass similar to that of LSA3. T-cell epitopes cross-reacting withP. yoeliiwere also demonstrated using peripheral blood lymphocytes from LSA3-immunized chimpanzees. In contrast, no cross-reactive epitopes were found in Plasmodium berghei. LSA3-specific human antibodies exerted up to 100% inhibition of in vitro invasion of P. yoeliisporozoites into mouse hepatocytes. This strong in vitro activity was reproduced in vivo by passive transfer of LSA3 antibodies. These results indicate that the homologous epitopes may be biologically functional and suggest that P. yoeliicould be used as a model to assess the antisporozoite activity of anti-LSA3 antibodies. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
