| Autor: |
Melle, C., Ernst, G., Schimmel, B., Bleul, A., Kaufmann, R., Hommann, M., Richter, K. K., Daffner, W., Settmacher, U., Claussen, U., Eggeling, F. von |
| Zdroj: |
Journal of Proteome Research; October 2005, Vol. 4 Issue: 5 p1799-1804, 6p |
| Abstrakt: |
We analyzed 74 cryostat sections of central gastric tumor, tumor margin, and normal gastric epithelium using ProteinChip Arrays and SELDI-TOF MS. One peak was significantly down-regulated in tumor tissue (P = 1.43 × 10-6) and identified as pepsinogen C using MS/MS analysis and immunodepletion. This signal was further characterized by immunohistochemistry. This work demonstrates that differentially expressed signals can be identified and assessed using a proteomic approach comprising tissue-microdissection, protein profiling, and immunohistochemistry. Keywords: gastric cancer • proteomics • biomarker • ProteinChip technology • SELDI • microdissection • immunohistochemistry |
| Databáze: |
Supplemental Index |
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