| Autor: |
Grossmann, J., Roos, F. F., Cieliebak, M., Liptak, Z., Mathis, L. K., Muller, M., Gruissem, W., Baginsky, S. |
| Zdroj: |
Journal of Proteome Research; October 2005, Vol. 4 Issue: 5 p1768-1774, 7p |
| Abstrakt: |
We present AUDENS, a new platform-independent open source tool for automated de novo sequencing of peptides from MS/MS data. We implemented a dynamic programming algorithm and combined it with a flexible preprocessing module which is designed to distinguish between signal and other peaks. By applying a user-defined set of heuristics, AUDENS screens through the spectrum and assigns high relevance values to putative signal peaks. The algorithm constructs a sequence path through the MS/MS spectrum using the peak relevances to score each suggested sequence path, i.e., the corresponding amino acid sequence. At present, we consider AUDENS a prototype that unfolds its biggest potential if used in parallel with other de novo sequencing tools. AUDENS is available open source and can be downloaded with further documentation at http://www.ti.inf.ethz.ch/pw/software/audens/. Keywords: mass spectrometry • de novo sequencing • spectra preprocessing |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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