| Autor: |
Brenot, Audrey, Trott, Daren, Girons, Isabelle Saint, Zuerner, Richard |
| Zdroj: |
Antimicrobial Agents and Chemotherapy; March 2001, Vol. 45 Issue: 3 p870-877, 8p |
| Abstrakt: |
ABSTRACTThe Leptospira interrogans ponAandpbpBgenes were isolated and characterized.ponAand pbpBencode the penicillin-binding proteins (PBPs) 1 and 3, respectively. There is little sequence variation between the PBP genes from two L. interrogansstrains (serovar icterohaemorrhagiae strain Verdun and serovar pomona strain RZ11). The deduced L. interrogansPBP 1 and PBP 3 protein sequences from the two strains shared over 50% similarity to homologous proteins fromEscherichia coli. It was demonstrated for strain Verdun that ponAand pbpBare transcribed individually from their own promoter. The ponAandpbpBgenes from both strains are separated by 8 to 10 kb and oriented such that their transcription is convergent. The L. interrogansPBP 1 and PBP 3 proteins were synthesized inE. coliand were modified with ampicillin using a digoxigenin-ampicillin conjugate. These data show that both genes encode functional PBPs. |
| Databáze: |
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