Construction of Human Chromosome 16- and 5-Specific Circular YAC/BAC Libraries byin VivoRecombination in Yeast (TAR Cloning)

Autor: Kouprina, N., Campbell, M., Graves, J., Campbell, E., Meincke, L., Tesmer, J., Grady, D.L., Doggett, N.A., Moyzis, R.K., Deaven, L.L., Larionov, V.
Zdroj: Genomics; October 1998, Vol. 53 Issue: 1 p21-28, 8p
Abstrakt: Transformation-associated recombination (TAR) in yeast was exploited for the selective isolation of human DNAs as large circular yeast artificial chromosomes (YACs) from two rodent/human hybrid cell lines containing human chromosomes 5 and 16. TAR cloning vectors containing the F-factor origin of replication were constructed for use in these experiments. Presence of the F-factor origin in TAR vectors provides the capability of transferring the YACs generated byin vivorecombination in yeast intoEscherichia colicells and propagating them as bacterial artificial chromosomes (BACs). A high enrichment of human versus rodent YACs was observed during isolation of human DNA from the rodent/human hybrid cell lines. Although <3% of the DNA content in the hybrid cells was human, as many as 75% of the transformants contained human YACs. In contrast to the standard YAC cloning method based onin vitroligation, no human/mouse chimeras were observed during TAR cloning. The constructed human chromosome 16 YAC library had approximately 2.6× coverage, represented by 4320 YAC clones with an average insert size of 80 kb. YAC clones generated from chromosome 16 were successfully converted into BACs by electroporation of DNA isolated from yeast transformants intoE. coli.The BAC clones represent ∼0.6× chromosomal coverage. Pilot YAC and BAC libraries of chromosome 5 have been also constructed. The chromosomal distribution of YAC/BACs from chromosome 5 and chromosome 16 was evaluated by fluorescencein situhybridization (FISH). The distribution of FISH signals appeared random along the length of each chromosome. We conclude that TAR cloning provides an efficient means for generating representative chromosome-specific YAC/BAC libraries.
Databáze: Supplemental Index
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