| Autor: |
O'Neill, G. L., Brazier, J. S., Magee, J. T., Duerden, B. I. |
| Zdroj: |
Anaerobe; August, 1996, Vol. 2 Issue: 4 p211-215, 5p |
| Abstrakt: |
The Anaerobe Reference Unit is the designated typing laboratory for isolates ofClostridium difficilefrom England and Wales. Presently isolates are typed by pyrolysis mass spectrometry. As this method cannot assign permanent type designations to strains, an alternative method has been sought. In this study, pyrolysis mass spectrometry typing was compared with a modified PCR ribotyping method for typing 70 clinical isolates ofC. difficilefrom various geographical locations throughout England and Wales. PCR ribotyping identified 17 types among the 70 isolates, with 40 (57%) isolates assigned to a single PCR type, termed type I. Pyrolysis mass spectrometry typing identified 20 different clusters among the 70 isolates, with 31 (44%) isolates clustering together as a single cluster, B, all of which belonged to PCR type I. Overall the methods were of similar discriminatory power and there was a good correlation between the results of both typing systems. The combined use of these methods has allowed the identification of a potentially widely dispersed clone ofC. difficile. |
| Databáze: |
Supplemental Index |
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