Crystallization and Preliminary X-ray Diffraction Studies on a Soluble Escherichia coliQuinone Oxidoreductase

Autor: Edwards, Karen J., Thorn, Jennifer M., Daniher, Jenny A., Dixon, Nicholas E., Ollis, David L.
Zdroj: JMB Online (Journal of Molecular Biology); July 1994, Vol. 240 Issue: 5 p501-503, 3p
Abstrakt: Single crystals suitable for X-ray diffraction studies have been obtained from a soluble Esherichia coliNAD(P)H-dependent quinone oxidoreductase (QOR) of molecular mass 35 kDa. Crystals were grown by the vapour diffusion method in the presence of NADPH and diffract strongly to beyond 2·0 Å. The space group was determined to be P212121with unit cell dimensions of a= 107·44 Å, b= 104·06 Å, and c= 77·45 Å. There is one dimer in the asymmetric unit.
Databáze: Supplemental Index