| Autor: |
Lakkis, Maha M., Bergenhem, Nils C.H., Tashian, Richard E. |
| Zdroj: |
Biochemical and Biophysical Research Communications; September 1996, Vol. 226 Issue: 1 p268-272, 5p |
| Abstrakt: |
The α-carbonic anhydrase (α-CA) gene family in mammals encodes 10 CA or CA-like proteins (CA I-CA X). Although the gene for human CA VII has been cloned and characterized, the corresponding protein has not previously been purified, and hence, the CO2hydrase activity of its product has not as yet been demonstrated. In this study, we have cloned the mouse CA VII cDNA in anE. coli,glutathione-S-transferase (GST) expression vector. The CO2hydrase activity of the expressed protein is about 4% that of the high-activity CAII isozyme, demonstrating that this evolutionarily highly conserved protein is a catalytically active member of this CA gene family. |
| Databáze: |
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