| Autor: |
Ghosh, R., Hoenger, A., Hardmeyer, A., Mihailescu, D., Bachofen, R., Engel, A., Rosenbusch, J. P. |
| Zdroj: |
JMB Online (Journal of Molecular Biology); May 20, 1993, Vol. 231 Issue: 2 p501-504, 4p |
| Abstrakt: |
Homogeneous detergent-solubilized B873 light-harvesting complexes from a carotenoid-less mutant of the purple non-sulfur bacterium, Rhodospirillum rubrum G9, were reassembled spontaneously into two-dimensional (2D) hexagonal arrays during extensive and controlled dialysis. As the complexes contain only 1 to 2 mol phospholipid per mol αβ dimer, the arrays formed by a self assembly process are primary due to protein-protein interactions. The hexagonal lattices were analyzed by negative stain electron microscopy and digital image processing. They exhibited a unit cell size of 12·3 nm, in close agreement with the particle diameter of the active photo-unit in native chromatophore membranes. The unit cell contains a central 15 nm stain-filled depression, embraced by a ring with an outer diameter of 10 nm. Copyright 1993, 1999 Academic Press |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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