| Autor: |
González-Zorn, Bruno, Catalan, Ana, Escudero, Jose A., Domínguez, Lucas, Teshager, Tirushet, Porrero, Concepción, Moreno, Miguel Angel |
| Zdroj: |
Journal of Antimicrobial Chemotherapy (JAC); September 2005, Vol. 56 Issue: 3 p583-583, 1p |
| Abstrakt: |
<it>Objectives and methods</it>: <it>armA</it> is a novel plasmid-borne 16S rRNA methyltransferase that confers high-level resistance to 4,6-disubstituted deoxystreptamines. Recently, we have isolated from a high-level broad-spectrum aminoglycoside-resistant <it>Escherichia coli</it> animal isolate a plasmid, pMUR050, that bore the <it>armA</it> gene. In order to elucidate the genetic basis for the spread of <it>armA</it>, we have determined the complete nucleotide sequence of pMUR050. <it>Results</it>: <it>armA</it> was borne by a complex transposon composite flanked by two direct repeats of IS<it>26</it>. The transposon composite included a class one integron with <it>sul1</it> for resistance to sulphonamides and <it>ant3″9</it> conferring resistance to spectinomycin–streptomycin, and a macrolide efflux pump and <it>mefE/mel</it> conferring high-level resistance to erythromycin. We identified in GenBank that another plasmid, pCTX-M3, from a Polish <it>Citrobacter freundii</it> human isolate, bore the same genetic structure, including <it>armA</it>. <it>Conclusions</it>: <it>armA</it> is present in human and animal isolates within a novel transposon composite. Further spread of <it>armA</it> between bacteria of diverse origin is to be expected. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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