| Abstrakt: |
G protein-coupled receptors mediate their biological responses through the generation of second messengers, such as cAMP. The down-regulation of their activity (desensitization) is carried out, in part, by the family of G protein-coupled receptor kinases, which phosphorylate activated receptors. The Gprk2gene in Drosophila melanogasteris a putative member of this family. The GPRK2 protein is expressed most abundantly in the ovaries and in the mushroom bodies, the brain region that is implicated in learning and memory in insects. Many of the genes that are involved in learning in Drosophila are members of a cAMP-signaling pathway and are also expressed in the mushroom bodies. These observations suggest that the Gprk2gene may be involved in a cAMP-mediated pathway. To investigate this possibility, we tested for a genetic interaction between Gprk2and dunce(which encodes cAMP-specific phosphodiesterase). A mutant allele of Gprk2,called gprk26936, has decreased fertility as a result of reduced levels of egg laying and hatching, and developing egg chambers display defects in the formation of anterior structures. Similarly, many alleles ofdunceare sterile, with an ovary phenotype that resembles gprk26936. Introduction of a single copy of a hypomorphic or null allele ofdunceinto the gprk26936background suppressed all of these defects to a significant degree. Suppression was also observed when a single copy of gprk26936was introduced into aduncebackground. Like mutants of rutabaga(which encodes a calcium/calmodulin-dependent adenylate cyclase), gprk26936has reduced levels of cAMP. Ovaries from gprk26936females contain about one third of the normal amount of cAMP. In addition, in every mutant combination where fertility is increased, cAMP levels are closer to wild type levels. These results suggest thatGprk2is functioning in a cAMP-signaling pathway and that the underlying basis of the interaction between Gprk2and dunceis a normalization of cAMP levels. |
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