| Autor: |
YU, PAULA K, YU, DAO-YI, ALDER, VALERIE A, SEYDEL, ULRICH, SU, ER-NING, CRINGLE, STEPHEN J |
| Zdroj: |
Experimental Eye Research; September 1997, Vol. 65 Issue: 3 p379-389, 11p |
| Abstrakt: |
The pivotal role of the endothelial cell in the regulation of vascular tone has been well demonstrated in many vascular beds, including the retina. However, in the retina, little is known about how the structural elements of the endothelial cells are arranged along the arborisation pathway from artery to vein, the nature of which has been linked to functional heterogeneity in other vascular beds. The relative vulnerability of the retina to vascular based diseases, and the heavy reliance on local regulation of the retinal vasculature makes an improved understanding of such local regulatory mechanisms of significant clinical importance. The present study focuses on identifying differences in endothelial cells along the arborisation pathway in the porcine retinal vasculature. Enucleated pig eyes were arterially cannulated and perfused with fixative followed by double staining for F-actin microfilaments (rhodamine phalloidin) and nucleic acid (YO-PRO-1). The intact retina was then viewed by confocal microscopy. The distribution of F-actin, vessel diameter, endothelial cell size and shape, nucleus size and shape, and position within the cell were determined as a function of location along the vascular tree. |
| Databáze: |
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