| Autor: |
Shevelev, Alexei B., Aleoshin, Vladimir V., Trachuk, Lesya A., Granovsky, Alexei E., Kogan, Yakov N., Rumer, Leonid M., Serkina, Anna V., Semenova, Elena V., Bushueva, Anastassia M., Livshits, Vitaly A., Kostrov, Sergey V., Shcheglov, Alexander S., Novikova, Svetlana I., Chestukhina, Galina G. |
| Zdroj: |
Plasmid; May 2000, Vol. 43 Issue: 3 p190-199, 10p |
| Abstrakt: |
The pLF1311 natural plasmid from Lactobacillus fermentum1311 was used to construct a single-replicon vector suitable for rapid cloning in a wide range of gram-positive hosts and Escherichia coli.The new vector is capable of conjugative mobilization from E. colito various hosts by conjugal transfer. The final vector (3.4 kb) showed a high segregational and structural stability and a high copy number. Glutamyl endopeptidase genes from Bacillus licheniformis(gseBL) and B. intermedius(gseBI) were cloned in both pLF9 and pLF14 vectors and introduced to B. subtilis.The yield of enzymes in the pLF-derived producers was 6- to 30-fold more than in the natural producers and reached 100–150 mg/L of mature protease. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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