| Autor: |
Funaoka, Kohsei, Shindoh, Masanobu, Yoshida, Koichi, Hanzawa, Motoaki, Hida, Kyoko, Nishikata, Satoshi, Totsuka, Yasunori, Fujinaga, Kei |
| Zdroj: |
Biochemical and Biophysical Research Communications; July 1997, Vol. 236 Issue: 1 p79-82, 4p |
| Abstrakt: |
p21Waf1/Cip1is one of the key regulatory proteins in cell cycle, terminal differentiation, and apoptosis. Its promoter was shown to be transactivated by the wild-type p53 protein as well as in a p53-independent manner. In this report, we demonstrate that E1AF, anets-related transcription factor, activates the human p21Waf1/Cip1promoter by interacting with theets-binding sites located close to the two previously identified p53-responsive elements. Northern blot analysis revealed that p21Waf1/Cip1and E1AF were correlatively upregulated in response to cisplatin treatment in SiHa cells. Transient expression assays demonstrated that E1AF can activate the p21Waf1/Cip1promoter-driven luciferase reporter gene in SiHa cells. The p21Waf1/Cip1promoter activity was also increased in p53-null Saos2 osteosarcoma cells, but was markedly reduced when theets-binding sites were deleted. These results indicate that E1AF positively regulates transcription from the p21Waf1/Cip1promoter in response to genotoxic stresses. |
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